Journal: Journal of Translational Medicine

Article Title: m6A methylation-modified ETV1 drives multiple myeloma progression and M2 polarization of tumor-associated macrophage through transcriptional activation of RBMS1

doi: 10.1186/s12967-026-07799-7

Figure Lengend Snippet: ETV1 promotes MM cell proliferation and cell cycle progression. ( A ) Cell viability was measured by CCK-8 assay. ( B ) Cell proliferation was determined by colony formation assay. ( C ) Cell cycle distribution was detected by flow cytometry. ( D ) The expression of cyclinD1 in MM cells was tested by western blot. Data are mean ± SD. ##, p < 0.01; ###, p < 0.001; ####, p < 0.0001

Article Snippet: The membranes were then incubated overnight at 4 °C with the following primary antibodies: human ETV1 (1: 1000 dilution, AP51197, Abcepta), human cyclinD1 (1: 5000 dilution, 26939-1-AP, Proteintech, Wuhan, China), human RBMS1 (1: 1000 dilution, 11061-2-AP, Proteintech) and human CCL2 (1: 500 dilution, 507277, Zen-bioscience, Chengdu, China).

Techniques: CCK-8 Assay, Colony Assay, Flow Cytometry, Expressing, Western Blot

Journal: Journal of Translational Medicine

Article Title: m6A methylation-modified ETV1 drives multiple myeloma progression and M2 polarization of tumor-associated macrophage through transcriptional activation of RBMS1

doi: 10.1186/s12967-026-07799-7

Figure Lengend Snippet: RBMS1 knockdown counteracts the effect of ETV1 overexpression on MM cell proliferation and M2 polarization of TAMs. ( A ) siRNAs targeting RBMS1 were transfected into RPMI8226 cells. After 48 h, the knockdown efficiency of RBMS1 in cells was verified by qPCR. ( B ) Cell viability was measured by CCK-8 assay. ( C ) Cell cycle distribution was detected by flow cytometry. ( D ) The expression of cyclinD1 in cells was detected by western blot. ( E ) The levels of CCL2 in the cell supernatant were determined by ELISA. ( F ) M2 polarization of TAMs was tested by Transwell co-culture system. M0 macrophages were seeded into the lower chamber, and MM cells were seeded into the upper chamber. After 48 h of co-culture, the expression of CD163 and CD206 in M0 macrophages were determined by qPCR. ( G ) The proportion of CD206 + cells and MFI in M0 macrophages were examined by flow cytometry. Data are mean ± SD. #, p < 0.05; ##, p < 0.01; ###, p < 0.001; ####, p < 0.0001

Article Snippet: The membranes were then incubated overnight at 4 °C with the following primary antibodies: human ETV1 (1: 1000 dilution, AP51197, Abcepta), human cyclinD1 (1: 5000 dilution, 26939-1-AP, Proteintech, Wuhan, China), human RBMS1 (1: 1000 dilution, 11061-2-AP, Proteintech) and human CCL2 (1: 500 dilution, 507277, Zen-bioscience, Chengdu, China).

Techniques: Knockdown, Over Expression, Transfection, CCK-8 Assay, Flow Cytometry, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Co-Culture Assay